Visualization of CRISPR-Cas9 complex

Program         Ph.D. (Systems Biosciences)

Course            MBSB 504 Techniques in Systems Biosciences

Lecture topic Visualization of CRISPR-Cas9 complex

Lecturer         Asst. Prof. Dr.Duangrudee Tanramluk

Date/Time      Tuesday 14 June 2022 (13.30 ̶ 15.30 pm) Room MB Building (Laboratory C410)

Learning Objectives:

    At the end of this lecture, students should be able to            

1. Describe the components of CRISPR-Cas9 structures

2. Create graphical illustration of CRISPR-Cas9 complexes

Lesson contents in brief:

Visualization of CRISPR-Cas 9 structures retrieved from bioinformatics databases, learn to create poster of the structure from Nishimasu H, et al.’s CRISPR-Cas9 paper


  1. Nishimasu, H., et al. (2014). “Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA.” Cell 156(5): 935-949. (

Learning organization:

1. Lecture

2. Hands on practice


            A Personal Computer with Microsoft Windows Operating Systems

Learning materials:

1. Discovery Studio, Photoscape, Powerpoint (download from: Google Class)

2. Handout

3. Powerpoint slides via website.

Access the Interactive Slides here!


1.Take a photo of physical model of the four helix bundle cytochrome b562, PDBID: 256b. Browse Protein structures in and if possible make your physical model and take picture with your name written underneath.

2.Learn to create poster of the structure from Nishimasu H, et al.’s CRISPR-Cas9 paper (Source:

3.Make protein structure animations and send the file to me.

Student Assessment: 

#1, #2, #3 above with the file name (StudentName_XXX.YYY)

Practical on hemoglobin (.jpg/.png) + Crispr-Cas9 poster (.gif/.pdf/.png/.jpg) + Protein animation (.gif/.mp4)

Please post the folder (containing 3 files per each student) to Google Class by June 18, 2022.

Returned Homeworks

Physical Models

CRISPR-Cas9 poster