MBSB504

Visualization of CRISPR-Cas9 complex

Program Ph.D. (Systems Biosciences)

Course MBSB 504 Techniques in Systems Biosciences

Lecture topic Visualization of CRISPR-Cas9 complex

Lecturer Asst. Prof. Dr.Duangrudee Tanramluk

Date/Time Tuesday 14 June 2022 (13.30 ̶ 15.30 pm) Room MB Building (Laboratory C410)

Learning Objectives:

At the end of this lecture, students should be able to

1. Describe the components of CRISPR-Cas9 structures

2. Create graphical illustration of CRISPR-Cas9 complexes

Lesson contents in brief:

Visualization of CRISPR-Cas 9 structures retrieved from bioinformatics databases, learn to create poster of the structure from Nishimasu H, et al.’s CRISPR-Cas9 paper

References:

Nishimasu, H., et al. (2014). “Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA.” Cell 156(5): 935-949. (http://www.sciencedirect.com/science/article/pii/S0092867414001561)

Learning organization:

1. Lecture

2. Hands on practice

Equipment:

A Personal Computer with Microsoft Windows Operating Systems

Learning materials:

1. Discovery Studio, Photoscape, Powerpoint (download from: Google Class)

2. Handout

3. Powerpoint slides via website.

Access the Interactive Slides here!

Assignments

1.Take a photo of physical model of the four helix bundle cytochrome b562, PDBID: 256b. Browse Protein structures in http://www.CATHdb.info/ and if possible make your physical model and take picture with your name written underneath.

2.Learn to create poster of the structure from Nishimasu H, et al.’s CRISPR-Cas9 paper (Source: http://www.sciencedirect.com/science/article/pii/S0092867414001561)

3.Make protein structure animations and send the file to me.

Student Assessment:

Assessment
#1, #2, #3 above with the file name (StudentName_XXX.YYY)

Practical on hemoglobin (.jpg/.png) + Crispr-Cas9 poster (.gif/.pdf/.png/.jpg) + Protein animation (.gif/.mp4)

Please post the folder (containing 3 files per each student) to Google Class by June 18, 2022.